Intracytoplasmic Sperm Injection (ICSI)
Typically it is used in case of low sperm count, poor sperm movement and even if when sperm are absent in ejaculate, it can be extracted from testis (testicular sperm extraction). With ICSI Treatment in India, the embryologist takes a single sperm and injects it directly into an egg.
The objective is being to increase the chances of fertilization and development of embryos used in conjunction with IVF. The treatment steps are similar to IVF except the step of fertilization, and may also be done if regular IVF treatment cycles have not achieved fertilization.
How ICSI is done in our fertility clinic?
As in IVF procedure, eggs are surgically removed which are normally surrounded by cluster of cells called cumulus corona cells, which needs to be removed before the sperm injection process takes place. Removal of these cells gives a clear insight of the eggs to an embryologist to access the maturity of the egg.
On the other hand, male partner need to provide his sperm sample (either retrieved surgically from testis or by masturbation) for the procedure. Fresh eggs are then placed in a dish under 200 or 400 magnification with an inverted microscope fitted with micromanipulator. The egg which is around 100 micron in width and is held by a glass holding pipette with a diameter of 40 to 50 micron usually on the left side. Sperms are first immobilized with chemical (PVP) and by hitting on the tail, loaded in the injection pipette.
Injection is done with very fine movements of micromanipulator and usually it is completed within a minute. After 16-18 hours of injection, fertilization is checked and it is the skill of embryologist which matters the most in whole procedure. Result: The fertilization rate with ICSI is around 85% (we should not expect 100% fusion of both the gametes, even though sperms are injected inside the eggs by us).